Using only a Beer Glass and a few other household items and ingredients.
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I've had a long, difficult week, so I've decided to pick something pointlessly
pathological for today. It's a remarkably goofy language called
How to make pumpkin pie
This makes one pie. You probably want two, so double everything.
PROCEDURE
PREHEAT oven to 425F. Set the rack to above the middle of the oven but not too high up.
Take a quick look around your home and chances are you’ll find at least one product with an ingredient simply described as “fragrance.” But what exactly does that mean and is there anything harmful in the ubiquitous chemical cocktails we refer to as fragrance? Maybe.
A couple of nights ago, I bought some toothpaste. At the risk of veering off into "in my day, we used sandpaper. And we liked it!" territory, there's a ridiculous variety of toothpaste.
Neat! That looks like it would be a pretty easy lab to do.
I gotta run to the drugstore and get some isopropyl alcohol. Otherwise I've got all the other ingredients.
Now I know what the heat is for, to denature the proteins. Ahhhhh. Now once you've got the DNA separated out how do you separate the base pairs?
Well, Tony, separating the helix can be done with heat as well, which is why he talks about being careful to not get it too hot. If you wanted to amplify this DNA sample you'd be using PCR. That involves heating a sample up, to the point of unzipping the helix, in the presence of a solution containing the 4 base pair elements and a thermally stable enzyme that will zip up the helix. You heat, separating the strands, then cool, allowing the enzyme to zip the free floating bases onto your two strands. Then you heat and cool again to come up with 4 strands. Repeat until you have the concentration you want.
A lot of times you're only interested in a small chunk of the genome, so you use more enzymes to trim a section of the strand out, then work at duplicating that.
So, to do that at home, you'd need to order the appropriate enzyme(s), and a small supply of the 4 bases. I'd be surprised if those weren't readily available. I've seen electrophoresis/PCR kits for kids at Fry's Electronics.
A friend of mine from YouTube made a similar video a while back. She's also got a bunch of other neat experiments (like how to measure the speed of light with marshmallows in your microwave).
Hmmm ... Onion-soap DNA milkshake ... [drools]
thats COOL!!
i wanna do it :)