Time for a therapy session.
Man I hate this. My cortisol levels must be through the roof. I tested my clones and got weird results. I was racking my brain all weekend trying to figure out why. Then yesterday I tested a positive control, and got negative results. I feel like I'm trapped in the twilight zone. (I would have rather been at the ASCB meeting.)
What to do?
One advice that I got long time ago - if things don't work anymore, throw away all your reagents and start all over. It's the best advice that anyone has ever given me.
So today I'm doing exactly that.
So, unfortunately, Nick was right: you thought that you were climbing up, when in fact you were climbing down.
That's the life of a postdoc...
That's so true it's sad.