By apalazzo on April 5, 2008. Well I finaly synthesized and purified the magic protein. Yesterday I used this protein in an experiment that was dictated to me by the ghost of Ockham. I should know the result this afternoon. Tags lab life Log in to post comments More like this So which one is your protein of interest, the higher molecular weight doublet or the larger band of low molecular weight protein? Log in to post comments The low one. It's a bit contaminated with that upper doublet, but if you consider that the bottom band has a lower MW and thus a higher mole/mass ratio then it's not so bad. Probably 95% pure. Log in to post comments More pure. Less magic. Log in to post comments
So which one is your protein of interest, the higher molecular weight doublet or the larger band of low molecular weight protein? Log in to post comments
The low one. It's a bit contaminated with that upper doublet, but if you consider that the bottom band has a lower MW and thus a higher mole/mass ratio then it's not so bad. Probably 95% pure. Log in to post comments
So which one is your protein of interest, the higher molecular weight doublet or the larger band of low molecular weight protein?
The low one. It's a bit contaminated with that upper doublet, but if you consider that the bottom band has a lower MW and thus a higher mole/mass ratio then it's not so bad. Probably 95% pure.
More pure. Less magic.