What Parrots Tell us About the Origin and Evolution of Birds

tags: parrots, Psittaciformes, evolution, molecular phylogeny, ornithology, Neornithes

One of the most contentious issues among scientists who study the evolution of birds is identifying precisely when the modern birds (Neornithes) first appeared. This is due to conflicts between the fossil record and molecular dating methodologies. For example, fossils support a Tertiary radiation whereas molecular dating methodologies suggest that the birds radiated in the early Cretaceous. But there is another way to address this discrepancy. Because the evolution of parrots and cockatoos reflects the evolution of Aves themselves, studying the psittaciformes offers compelling insights into this mystery. Further, because psittaciformes generally are not migratory and because they tend to occupy discrete ranges, their ancient patterns of diversification are easier to discern than for many other taxonomic orders of birds that have dispersed widely.

To this end, an international team of researchers, headed by Timothy Wright, a professor at New Mexico State University and a researcher at the Smithsonian Institution in Washington DC, collaborated to study the early evolution of the parrots. To do this work, they sampled individual representatives from 69 of the 82 extant genera of parrots and eight additional avian orders that have been idnetified as outgroups, for a total of 77 avian taxa. Outgroups are defined as those taxa that possess a character, such as a hooked beak or zygodactyl toes, that is likely to have been ancestral to the parrots.

Wright and his colleagues chose the Falconiformes (Falcons), Passeriformes (songbirds), Columbiformes (pigeons and doves), Cuculiformes (cuckoos), Piciformes (woodpeckers), Coraciiformes (the "near passerines", such as kingfishers, etc), Strigiformes (owls), and Coliiformes (mousebirds) as outgroups because each was considered an ally of the parrots at one time or has been identified as a sister group in recent molecular phylogenetic work [for example, see here].

The team sequenced and analyzed 3,915 basepairs (bp) of DNA per individual, consisting of 1,668 bp from mitochondrial DNA (mtDNA) that encodes two genes, and another 2,247 bp from nuclear (chromosomal) introns. Using these data, they used Maximum Parsimony analysis to recover this cladogram that describes the relationships between the 69 parrot genera and the eight outgroups (figure 1);

The team then used Bayesian analysis to reconstruct the evolutionary history of the parrots and recovered this phylogram, which describes very similar relationships to those in the above cladogram (figure 2);

The team then estimated divergence times using both two different analytic methods that relied upon incorporation of one of the two contrasting calibration dates (either based on fossil data and molecular data) (figure 3);

When the team used the molecular calibration date of 82 million years ago (MYA), they found that the evolutionary split between the cockatoos and the remaining psittaciforms occurred prior to the K/T boundary at 65 MYA. Subsequent parrot lineage radiations then occurred between 60 and 65 MYA, which corresponds to the initial separation of Australia and New Guinea from East Antarctica. According to this estimate, diversification into the major parrot subclades was complete by 30 MYA, by which point Australia, Antarctica, and South America were completely separated.

On the other hand, when the team instead used 50 MYA (fossil data) to calibrate the diversification in basal parrot lineages, they found that the split between the cockatoos and the remaining psittaciforms occurred 45.04 MYA -- just prior to the time Australia is thought to have separated from Antarctica. Radiation of Neotropical parrot lineages is estimated to have occurred starting 33 MYA, a period roughly coincident with the separation of South America from West Antarctica.

These results support the hypothesis that Australasia was probably where the parrots originated. This hypothesis is based in the high number of genera endemic to the region, several of which are rooted at or near the base of the psittaciform tree. Further, the basal split between the New Zealand taxa, Nestor and Strigops, suggests that this origin occurred while New Zealand and Australasia were still part of the Gondwana supercontinent during the Cretaceous period. Additionally, other recently published analyses using similar methods suggest that both Passeriformes and Columbiformes originated in Gondwana during the Cretaceous.

Other interesting findings include the confirmation that the cockatoos are a monophyletic group -- as are the lories (Loriinae), whose sister groups include Melopsittacus, Cyclopsitta, and Psittaculirostris -- a finding that agrees with my data (unpublished). Unsurprisingly, these data indicate that the remaining parrots form a polyphyletic jumble, with the exception of the tropical African parrots and the Neotropical Arini, each of which were recovered as separate well-supported clades.

The team also found that their various phylogenies did not produce any consistent placement of the eight outgroups as sister to the parrots, reinforcing the idea that the parrots and cockatoos do not have any close sister relationships with other modern birds.

This research indicates that Aves appeared during the Cretaceous, and that parrots are an ancient lineage without any close evolutionary relationships. Additionally, these data also support the hypotheses that there are several separate and well-defined clades of parrots, particularly cockatoos and the lories, while the remainder of the parrots need some serious phylogenetic work to be done -- work that I would absolutely love to devote my life to completing.

Source:

T. F. Wright, E. E. Schirtzinger, T. Matsumoto, J. R. Eberhard, G. R. Graves, J. J. Sanchez, S. Capelli, H. Muller, J. Scharpegge, G. K. Chambers, R. C. Fleischer (2008). A Multilocus Molecular Phylogeny of the Parrots (Psittaciformes): Support for a Gondwanan Origin during the Cretaceous. Molecular Biology and Evolution, 25 (10), 2141-2156 DOI: 10.1093/molbev/msn160.